Bean Yellow Mosaic Virus on Broad Bean Plants in Libya -II. Purification and electron microscopy

Abstract

A wide spread mosaic virus disease affecting broad bean plants around Tripoli was identified as being caused by bean mosaic virus infection. This was based upon the results obtained from the study of the host range, physical properties and transmission of the virus. Broad bean, bean, lupins, and C. Amaranticolor Coste and Reyn were found to be hosts of this virus. The thermal inactivation point of the virus isolate was found to be between 60 62 C. The virus withstood a dilution of 1 JOO but not 1:1000. It lost its infectivity when stored in a crude juice at room temperature for 24 hours but not for 16 hours. It was readily transmitted mechanically and by Aphis gossypii Glover and Macrosiphum pisi (Harris).