Isolation and Identification of Potato Virus X From Potatoes at Ain-Zara, Libya

Abstract

A virus was isolated from naturally infected potatoes growing in a potato field at Ain-Zara, Libya. The young leaves showed mild mosaic, whereas the older ones developed general yellowing. The virus was mechanically transmitted to 19 out of 24 tested plant species belonging to six families. It has TIP of 70-75°C, DEP of 10-5 — 10-6 and LIV of 60 days. The virus was purified by differential and density gradient centrifugation. Its particles are slightly flexuous. The virus is immunogenic and has a serological relationship with potato virus X (PVX) but not with eight other potexvir-uses and two carlaviruses. It is concluded that the virus under study is PVX.