Effect of basal medium and growth regulators on Micro propagation of Bauhinia purpurea L.

Abstract

This study was conducted during the years 2016 – 2017 in Plant Tissue Culture Laboratory at the Agriculture Research Center in Sidi El-Massri Station,Tripoli . The objective of this study was to establish a microproagtion protocol for Bauhinia purpurea L.   Shoots were taken from in vitro grown seedlings after 45 days of culture and prepared into single nodes of 1.0cm length carrying a single bud, cultured on Murashige and Skoog medium (MS) or Loyd and McCown medium (WPM) containing different concentrations of Benzyl adenine (BA)  alone  or in combination  with 1 mgL^-1 Naphthalene acetic acid (NAA) for multiplication. For rooting, shoots which were produced from single nodes, were cultured on ¼ , ½ or full strength MS basal salts media supplemented  with different concentrations of Indole butyric acid (IBA) or NAA with or without  0.3% activated charcoal. The results showed that   MS   Medium   containing 1.5 mgL^-1 BA alone or in combination with 1 mg L^-1  of NAA   gave the best results based on shoot number and  shoot length  (4.5 and 7.3 cm) and highest leaf number.  Rooting results showed that, shoots which were cultured on ½ MS medium supplemented with 5 mgL^-1 IBA   containing 0.3% activated charcoal had the highest rooting percentage (88.8 %), number of roots (6.6) and length of root (3.6cm). Plantlets were successfully acclimatized in a sterile growing mixture composed of soil with peat moss at 1:1 (v\ v) with a survival rate 75% after 6 weeks.