A survey of distribution and physiologic races of wheat yellow rust (Puccinia striiformis f. sp. Tritici) in north eastern of Syria during 2021 and 2022

Abstract

This study was conducted during the years 2016 – 2017 in Plant Tissue Culture Laboratory at the Agriculture Research Center in Sidi El-Massri Station,Tripoli . The objective of this study was to establish a microproagtion protocol for Bauhinia purpurea L.   Shoots were taken from in vitro grown seedlings after 45 days of culture and prepared into single nodes of 1.0cm length carrying a single bud, cultured on Murashige and Skoog medium (MS) or Loyd and McCown medium (WPM) containing different concentrations of Benzyl adenine (BA)  alone  or in combination  with 1 mgL^-1 Naphthalene acetic acid (NAA) for multiplication. For rooting, shoots which were produced from single nodes, were cultured on ¼ , ½ or full strength MS basal salts media supplemented  with different concentrations of Indole butyric acid (IBA) or NAA with or without  0.3% activated charcoal. The results showed that   MS   Medium   containing 1.5 mgL^-1 BA alone or in combination with 1 mg L^-1  of NAA   gave the best results based on shoot number and  shoot length  (4.5 and 7.3 cm) and highest leaf number.  Rooting results showed that, shoots which were cultured on ½ MS medium supplemented with 5 mgL^-1 IBA   containing 0.3% activated charcoal had the highest rooting percentage (88.8 %), number of roots (6.6) and length of root (3.6cm). Plantlets were successfully acclimatized in a sterile growing mixture composed of soil with peat moss at 1:1 (v\ v) with a survival rate 75% after 6 weeks.