Bean Yellow Mosaic Virus on Broad Bean Plants in Libya I. Identification of the causal agent

Abstract

Broad bean plants infected with bean yellow mosaic virus (BYMV) were used for the electron microscopy of the casual agent. The dip method revealed the presence of a few elongated-rod-shaped particles. Preparations from infected tissues using organic solvent mixture of ethylene glycol monethyl ether and ethylene glycol monobutyl ether and using borate buffer resulted in producing fairly clean preparations containing flexuous elongated virus-like particles with a length of 729 to 750 mu. This purified preparation was found to be infectious. However, the purified preparations from infected tissues following the phosphate buffer and differential centrifugation method yielded no virus-like particles when examined with the electron microscope.