Libyan Journal of Veterinary and Medical Sciences

Comparative Assessment of SepsiTestTM Platform to BactScreenTM and “in- house” MGB-based All Bacteria Assay for Detection of Bacteraemia in Whole Blood Samples

2024-04-28T19:53:09+00:00

Several molecular techniques that are commercially described in the literature for early detection of blood stream infection in attempting to overcome the limitations of the gold standard blood culture. SepsiTestTM is aCE marked commercial platform that has been described in the literature. However, there were no studies addressing the accuracy of the test as a diagnostic platform for detection bacteraemia in whole blood samples. The study, conducted to investigate and discuss the strategy of SepsiTestTM in comparisonto our previously validated real-time PCR based assays (BactScreenTM) and the previously published “in-house” minor groove binder (MGB)-based all bacteria assay. The three assays showed different sensitivity patterns for detecting bacteria in blood stream pathogens in favouring of using BactScreenTMtest. SepsiTestTM could be valuable but their lowest sensitivity in addition to their use of the unspecific SYBR Green fluoresce dye that question its diagnostic accuracy could be used as a last choice molecular diagnostic technique for detection of bacteraemia in whole blood samples. However, SepsiTestTM strategy rather, may provide useful diagnostic tool for detecting live pathogens in food technology.

Soil contamination with Toxocara spp. Eggs in the Public Parks of Tripoli City, Libya

2024-04-28T20:00:17+00:00

Toxocara spp. is a highly prevalent nematode that parasitizes the gastrointestinal tract of dogs and other canids. The eggs shed in the host’s feces can survive for a very long time in the environment and infect other canids and humans. Little information is available on the prevalence of and the risk factors associated with toxocariasis in Libya. To assess soil contamination with Toxocara spp., one hundred and five soil samples from public parks in different localities in Tripoli were examined by Dunsmore modified technique. The overall prevalence of Toxocara spp. in soil from public parks was 59.0% (62/105 samples). Our findings show a widespread soil contamination in public parks with Toxocara spp. eggs. Combined, these are a potential public health risk.

The Effect of Pesticide Chlorpyrifos (DursbanR) on Sperm Parameters and Testicular Tissue in Mice

2024-04-28T20:05:43+00:00

Environmental pollution by the pesticide chlorpyrifos has been documented as the most commonly detected pesticide in food and water posing a potential hazard to human health. Therefore, the present study was conducted to determine the effect of pesticide chlorpyrifos on sperm parameters and testicular tissue in mice. Sperm count, sperm motility and sperm morphology parameters are very important semen characteristics and are strong predictors of male fertility. The reproductive toxicity of the pesticide chloropyrifos was studied in adult male mice via intraperitoneal administration of chlorpyrifos (20 mg/kg) on alternate days for a period of 15 days. The results of this study showed a decrease in all sperm parameters, especially sperm motility and histological lesions of testicular tissue. The histological lesions emphasize the positive correlation between cytogenetic damage and abnormal sperm parameters. These results support the hypothesis that exposure to pesticides may be associated with decreased semen quality and hence infertility.

Incidence and Antimicrobial Susceptibility of Salmonella Carrier among Apparently Healthy Camels in Some Libyan Regions

2024-04-28T20:16:05+00:00

Our study were planned to investigate the incidence of salmonella species among apparently healthy camels. One hundred and twenty one (121) samples were collected from camel’s feces (79 from Egdabya and 42 from El-mackeli) in Libya. Bacteriological examination of samples showed that the incidence of positive fecal samples were 16 (13.2%) according to their culture and morphological characteristics. Biochemical and serological identification revealed the following serotypes: six of S. typhimurium with incidence of (37.5%), four of S. entretids (25%), three of S. frintrop( (18.8%), in addition to three isolates were not identified. Anti- biotic sensitivity of isolated serotype showed that serovars were most sensitive to Ampicillin, Amoxicillin/Clavulanic acid (all isolates) and Chloramphenicol which could be used for treatment of salmonellosis in camels. The epidemiology and zoonotic significance of salmonella infection were discussed in

The effect of Indoxacarb on blood parameters and liver tissues in Mice

2024-04-28T20:19:24+00:00

This study was conducted to evaluate the effect of Indoxacarb (Avant), an oxidiazine compound on hematological, some biochemical parameters of liver functions and histological changes in the liver. Sixteen albino adult mice were divided into two groups (n=8). Group1 (control group). Group 2 injected intraperitoneally with 90 mg/kg indoxacarb for a period of two weeks. Hematological investigations revealed a significant decrease in red blood cells count (RBC), haematocrit, mean corpuscular volume (MCV) and hemoglobin content (Hb) compared to the control group, whereas, white blood cells (WBC) and platelets counts a highly significant increase. Biochemical analysis illustrated that indoxacarb caused a marked elevation of alanine amino transferase (ALT) and considerable decrease in the total protein and albumin. Microscopic examination of liver sections illustrated that treatment with 90 mg/kg indoxacarb led to histoarchitectural changes of the liver including focal aggregations of lymphocytes, necrosis, degeneration of hepatocytes, inflammatory cells infiltration in portal tracts, pyknosis, karyolysis and sinusoidal dilatation. These results concluded that short period of exposure to indoxacarb may have toxic effects on public safety.