Effect of Storage Time and Temperature on the Stability of some Hepatobiliary Enzymes from Camel Serum

المؤلفون

  • Rehab Walli Department of Biochemistry and Clinical Biochemistry, Faculty of Pharmacy, University of Tripoli, Libya
  • Rabea Almosrati Department of Biochemistry and Clinical Biochemistry, Faculty of Pharmacy, University of Tripoli, Libya
  • Elham Omran Department of Biochemistry and Clinical Biochemistry, Faculty of Pharmacy, University of Tripoli, Libya
  • Raja Saleh Department of Biochemistry and Clinical Biochemistry, Faculty of Pharmacy, University of Tripoli, Libya
  • Arej Etbugh Department of Biochemistry and Clinical Biochemistry, Faculty of Pharmacy, University of Tripoli, Libya
  • Suad Essameie Department of Biochemistry and Clinical Biochemistry, Faculty of Pharmacy, University of Tripoli, Libya

الكلمات المفتاحية:

Serum enzyme activity; Storage time; Storage temperature.

الملخص

Camels can survive in the desert without food and water for a few days because of their unique biology. The
current study was performed to determine the effect of storage time and temperature on serum enzyme activities
of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) in young
female camel. The sera were stored at 4°C, 25°C and 40°C for 0, 1, 2, 3,7,11, and 15 days, then the activities
of these enzymes were determined. Our study revealed that the activity of AST, ALT, and ALP did not change
over 15 days when stored at 4°C or at room temperature. Interestingly, at 40°C deceased stability of AST was
apparent after 2 days, whereas, ALT and ALP show no loss of activity over 11 days. From these results it is
therefore advisable to consider stability of each serum hepatobiliary enzymes for different animals separately
before preserving sera samples to get more valid and reliable results.

التنزيلات

منشور

2024-09-13
#