Utilizing Marker-Assisted Selection (MAS) for Morphological Traits in the F3 Generation of Tomato (Solanum lycopersicum).

Main Article Content

Amani Emsaed
Aysam M. Fayed
Khaled Elmeer

Abstract

The use of molecular markers has become a pivotal component of molecular breeding programs in tomato. In the third generation of this research, two lines derived from the crossbreeding of commercial hybrids were subjected to detailed analysis using molecular markers by ten RAPD-based primers. Among these primers, six (OPA01, OPA05, OPA07, OPA08, OPA09, and OPB10) successfully amplified targeted DNA regions. Significantly, primer OPA07 uniquely linked specific traits in the first line to distinct bands of 280 and 180 base pairs. In the second line, all six primers achieved amplification, with four primers (OPA07, OPA08, OPA09, and OPB10) associating selected traits with distinct bands of 450, 500, 350, and 450 base pairs, respectively. These bands are likely to serve as marker-assisted selection (MAS) tools, facilitating the selection of plants with desired phenotypic traits in tomato breeding. These markers are expected to greatly improve marker-assisted selection techniques and potentially reveal novel genetic resources, thus enriching the genetic diversity within tomato breeding programs for future advancements.

Article Details

How to Cite
Emsaed, A. ., Fayed, A. M. ., & Elmeer, K. . (2025). Utilizing Marker-Assisted Selection (MAS) for Morphological Traits in the F3 Generation of Tomato (Solanum lycopersicum). The Libyan Journal of Agriculture, 30(1), 59–68. Retrieved from https://uot.edu.ly/journals/index.php/ljagric/article/view/1764
Section
Articles
Author Biographies

Amani Emsaed

University of Omar AlMukhtar - Libya

Aysam M. Fayed

 

University of Sadat City – Menoufia - Egypt

Khaled Elmeer

Department of Horticulture- Faculty of Agriculture- University of Tripoli - Libya