Tissue culture is from the compulsory
courses. In general, it concerns of the propagation techniques of in vitro.
Its elements are: general introduction, the historical development, the techniques and applications - advantages and
problems - tissue culture laboratories, propagation methods and influencing
factors - Growth regulators and their effects, tissue culture techniques:
apical meristem culture - zygotic embryo culture - thin cell layer technique, isolation,
cultivation and fusion of protoplasts and plant improvement, production of
haploid and polyploidy plants- Methods for ploidy detection, genetic and
phenotypic variations - mutations Induction, callus initiation and cell
suspension, production of secondary compounds – bioreactors, micropropagation
and its stages - autotrophic propagation, bioadaptation – acclimatization - plant
tissue quality - technology integration from laboratory to field, methods of
preserving genetic resources , and, The role of tissue culture in genetic
engineering.
1-Gaining information
about theoretical and physiological fundamentals of plant tissue culture
2- Learn about plant
tissue culture techniques
3- Understanding modern
techniques used in plant improvement
4- Gaining information on production of secondary compounds
5- Learning the modern
methods of preserving genetic resources
