Abstract
propolis is a substance collected by bees from the leaf buds of several different kinds of trees. The chemical composition and biological activity of propolis has been reported to be variable between different geographical regions. The current study designed to investigate of chemical constituents and antioxidant and antimicrobial activity of propolis sample collected from area around Tripoli. The phytochemical analysis of the propolis extract display presence of compounds belonged to phenolic, flavonoids, terpenoids, phytosterol and coumarine. The results of TLC illustrate DPPH free radical scavenging potential of EEP as the color changed from violet to yellow. The 1mg/ml of EEP showed significant antibacterial effect against S. aureus, Ps. aeruginosa and S. poona, and lower effect against MRSA, K. pneumonia and E. coli However other concentration had lower activity against tested bacteria.