المهام

 Dr/ Hania Aween, I have successfully completed my PhD in 2019 with the title (Transposon mutagenesis and characterisation of the selenite reduction pathway in Methylosinus trichosporium) in the laboratory of Prof Tom Smith at Sheffield Hallam University in UK. During my PhD I worked as a laboratory demonstrator, teaching practical skills to undergraduate students from 2017 until 2020 for undergraduate laboratory classes at Sheffield Hallam University, and have received positive feedback about my teaching skills from academic staff in charge of the practicals. Having finished PhD, I worked in COVID 19 testing during the pandemic with PerkinElmer Company in Newport in UK, the title of my position was Clinical Laboratory Scientists RNA Extraction, and PCR.  

Lecturer at the University of Tripoli, Faculty of education, Qasr Bin Ghashir, Biology Department, from 2008 until now. I have taught the following subjects;

•  Botany 1
•  Botany 2
• Molecular biology

المسيرة المهنية

·       Lecturer in the department, Head of Biology Department 2008 – 20012.

الاهتمامات البحثية

I have a wide range of skills of microbiology and molecular biology, including aseptic technique for growth of E. coli and methanotrophic bacteria, studies of bacterial physiology, PCR, plasmid purification, transformation and conjugation and gel electrophoresis. I have experience of a range of protein analysis methods, including proteolysis and SDS-PAGE. During my PhD project, I obtained and analysed a number of bacterial genome sequences using Mauve and Blast. During my PhD, I was the only researcher in the laboratory performing genetic work on methanotrophs, so I learned to become independent in my work and to become proficient in new techniques largely on my own. This included developing methods for screening methanotroph mutant libraries for selenite reduction activity, which I used to screen to 5,500 mutants

النشاطات الخارجية

This study aimed to identify the genes responsible for selenite pollution remediation in the methane-oxidizing bacteria Trichosporium OB3b. It was proposed to achieve this by creating a library of mutants inactivated in different genes and screening these mutants to see which were deficient in treatment reactions and thus identify the genes involved. Plasmid pSAM_Rl, which contains marine transposons and was developed for use in Rhizobiaceae, was introduced into Trichosporium by conjugative transfer of the plasmid from E. coli SM10 λpir. The strain from the conjugation was resistant to kanamycin (KM) (strongly indicating that it contained the plasmid) and resistant to nalidixic acid (confirming removal of the E. coli donor strain). The presence of transposons and their distribution around the chromosome were confirmed by genome sequencing of 18 randomly selected clones.