تقييم قدرة بعض أنواع البكتيريا المعزولة من البيئة المحلية على إنتاج إنزيم السليولاز تحت ظروف تنمية مختلفة

Abstract

This study focused on the isolation of bacteria and evaluate their ability to degrade cellulose and the effect of some factors to improve enzymatic degradation, included the effect of temperature, pH concentration and some nutritional additives, out of 10 isolates of bacteria, the results showed that, isolate (BA.10) showed the highest in the production of cellulase enzyme, and was identified as Pseudomonas fluorescence. The bacterial isolate exhibited a significant difference in their response to temperature and the influence of hydrogen ion concentration on cellulase enzyme activity. Additionally, the study evaluated the effects of several nutritional additives, including maltose, peptone, and powdered moringa leaves and stems, on cellulase production. Results also showed that the addition of maltose significantly increased the percentage of reducing sugars compared to the control. Furthermore, moringa leaf powder was found to enhance the bacterial isolate's ability to degrade cellulose relative to the control, whereas moringa seed powder did not demonstrate any significant effect on enzymatic degradation Compared to the control.