Department of Microbiology & Immunology

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5

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Department of Microbiology & Immunology has more than 5 academic staff members

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Dr. Basma Mohamed K Doro

بسمة دورو هي احد اعضاء هيئة التدريس بقسم الاحياء الدقيقة والمناعة بكلية الصيدلة. تعمل السيدة بسمة دورو بجامعة طرابلس كـاستاذ مساعد منذ 2017-04-15 ولها العديد من المنشورات العلمية في مجال تخصصها

Publications

Some of publications in Department of Microbiology & Immunology

In vitro Antibacterial Activity of Ficus carica L. (Moraceae) from Libya.

Aims: The aim of the study was to assess the antibacterial effect of Ficus carica extracts against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, & Staphylococcus aureus. Study Design: Evaluation of antimicrobial activity using Cup-cut agar method. Place and Duration of Study: Microbiology Research Laboratory, Department of Microbiology and Immunology, Faculty of Pharmacy, University of Tripoli, from October 2015 to March 2016. Methodology: The leaves and stem part extracts of Ficus carica plant were prepared using maceration method. The antibacterial activities of the extracts were evaluated using Cup-cut agar method to determinate inhibitory zone diameters in millimeters of the plant extracts against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The measurement of exponential bacterial growth curves was used to determine the type of growth pattern spectrophotometrically at 600 nm. Furthermore, plate count methods were also used to enumerate the bacterial count and to determine the percentage of inhibition as well as IC50. Results: The results of this study showed that Ficus carica extracts used, exhibited antimicrobial activity against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. The maximum zone of inhibition against S. aureus (27± 0.04mm, p
Basma Mohamed K Doro(1-2018)
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Blood donors status of HIV, HBV and HCV in Central Blood Bank in Tripoli, Libya

virus infection continues to be an important public health concern with regard to blood transfusion in Libya and in Africa. This concern is related to the screening test. Objectives: The main aim of this study to investigate the blood donors samples for HIV, HBV and HCV infections in Tripoli-Libya, North Africa during the first five months of 2015. Methods: The total of 686 blood samples obtained from healthy blood donors who attended Tripoli’s central blood bank, were tested for HBsAg, HCV and HIV using the VITROS® 3600 Immunodiagnostic System. Results: From the 686 samples examined, the frequency of HBsAg positive cases was 0.8%, the number of anti-HBc positive samples was found to be particularly high in the age group 29 and 36 years (p= 0.0001). The number of anti-HBc positive samples was found to be particularly high in the age group 30-39 years (p= 0.01). Most occupation that had positivity with anti-HBc and HBV-DNA were free workers and was less in students. Most positive cases were from east of Tripoli the capital (Tagora, Soq-Aljomaha). Conclusion: The frequency of HBsAg positive blood donors and anti-HBc among this sample was 0.8% and 0.7% respectively, which is low compared with the international findings. The current study estimated the expected exclusion rate of anti-HBc and HBsAg positive donated blood, as this would be an important factor to consider before donation. arabic 16 English 63
Basma Mohamed K Doro(9-2015)
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Phytochemical Screening and Antibacterial Activity of Libyan Globularia alypum

Aims. The aim of this study was to assess the phytochemical screening and antibacterial effect of the aerial parts of G. alypum cultivated in Libya. Methods. The methanolic extract of G. alypum (at concentrations 100 mg/ml, 300 mg/ml, 500 mg/ml and 700 mg/ml) were tested against 13 different strains of standard bacteria (ATCC) by four different methods; paper disc diffusion method, well diffusion method, broth dilution method and finally study the effect of extract on growth curve of bacterial cell were studied. Results. The results of phytochemical screening revealed the presence of flavonoids, phenols, reducing sugars, tannins, saponins, coumarins, steroids, terpenoids, carotenoids, antraquinones and glycosides. While the results of antibacterial tests showed remarkable inhibition of the bacterial growth, with maximum inhibition on growth of Methicillin Resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus. Moderate effect on growth of Enterococcus faecalis, Bacillus subtilis, Aeromonas hydrophila and Salmonella poona. While, the lowest inhibition showed in Yersinia enterocolitica and Listeria monocytogenes. However, the extract did not have any effect on Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella typhimurium and Klabsiella pneumonia. Conclusion. The methanolic extract of Globularia alypumaerial parts contain the most important constituents with positive results during phytochemical screening, and have antibacterial activity against gram positive and gram-negative bacteria.
Basma Doro, Abdulruzag Auzi, Suad Shanab(5-2021)
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