Abstract
Abstract This article builds a morphometric framework for the analysis of dental pulp fibroblast evolution during tooth development. The morphometric description concentrated on the parameters such as the area automatically extracted by a color staining technique. The designed framework operated at inter- and intragroup levels. It is generally accepted that the effect of extensive tooth movement on the dental pulp in adolescents is reversible and that it has no long-lasting effect on pulpal physiology. However, it is not clear yet if the same conclusion is also valid for adult subjects, with special emphasis on cell density, inflammatory cells, vascularity, and odontoblasts. Dimensions of dentin and the pulpal horns was determined and related with the duration of exceed force application and age ware evaluated. We found that the force application led to long-lasting or irreversible changes in pulpal tissues. In conclusion, the excess of tooth movement per se has no long-lasting or irreversible effect on pulpal tissues, neither in the young nor in the adult animals.