Abstract
Limb regeneration remains a challenge in medicine. Unlike some animals, humans lack this ability. This review investigates promising approaches to human limb regeneration and their potential for clinical translation. We analyzed 45 studies from 1,245 records published between 2010 and 2025, identifying three key strategies: Bioelectric signaling modulation, which successfully induced limb regrowth in frogs, including the development of webbing, fingers, and nerves; Tissue engineering integrates scaffolds and cells, achieving clinical restoration of motor functions by up to 60%. And the regenerative comparative studies of species revealing mechanisms found in axolotls still lack translation to mammals. Even with the challenges posed by the intricate design of mammalian electrical signaling, tissue integration, differences between species, and many more, these techniques embody promise towards devising therapies focused on functional limb regeneration that would surpass prosthetics. It is crucial to conduct more research to determine the optimal approach tailored for human use.