Abstract
We investigated honeybee populations of A. mellifera in Saharan and coastal locations in Libya to fill the North Africa gap of biogeography and distribution of honeybees, morphologically and using mtDNA analysis. It was found that Libyan honeybees are different, morphologically and genetically, from adjacent subspecies; and majority of Libyan bees (92%) belong to the oriental evolutionary lineage (O). As well as it was found a local impact of imported European honeybees. Further studies may to name the Libyan bees as a separate subspecies.