Abstract
This study was to determine if there is a difference in virulence between the two Salmonella enteritidis isolates from both laying hens and murine fecal pellets infesting the same layer farm by quantitative measurement of invasiveness using in vivo chicken intestinal loop model. Samples were collected from both live birds and patches of murine fecal pellets that infest the same henhouse. The presence of Salmonella spp. in collected samples was assessed by performing the pre-enrichment and enrichment cultures followed by staining, biochemical tests, and serological identification. The tested serotype demonstrated intracellular log10 counts was not significantly different from each other and from that of the reference strain only because of the allocation of intestinal loop position. This could explain the persistence of salmonella in poultry houses with the same capability of invasion restored from contaminated murine fecal pullets.