Precipitants and additives for membrane crystallization of lysozyme

Date

2024-1

Type

Article

Journal title

Author(s)

MOHAMED SALEH OMAR EL-BOURAWI

Abstract

crystallization technique that has proven to be superior in producing good crystal forms under operating conditions that are not appropriate to perform the crystallization process by other traditional techniques. In this work, static membrane crystallization was carried out on lysozyme, with hollow‐fiber microporous hydrophobic membranes. Numerous precipitant and additive types and concentrations were employed in the crystallization processes in order to select the most appropriate precipitant and additive types and to find their corresponding concentration levels that can yield the best crystal forms. The crystallization processes were analyzed in two ways: firstly, by evaluation of the transmembrane fluxes obtained by using different precipitants and additives; secondly, by utilization of the images and results obtained from the micrography and IR spectra in … إجمالي الاقتباسات تم اقتباسها في عدد: 11

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