Abstract
During January 2023, three broiler flocks had increased mortality up to 25-50 %, Paleness and haemorrhages in the liver appeared in birds that were 23 to 27 days old. The total number of birds were 12,000, 16000 and 22000 in the first, second and third flock, respectively. Ten Samples from the liver and kidneys were collected for histopathological examination from each flock, and liver, spleen and caecal tonsils were collected for DNA extraction, Real-time PCR and sequencing. Histopathological examination of the liver revealed hemorrhages, fatty changes and coagulative necrosis, presence of intranuclear eosinophilic inclusion bodies in hepatocytes and karyorrhexis of the nucleus and multiple perivascular infiltration of pleomorphic lymphocytes. The kidney showed subcapsular hemorrhages and infiltration of lymphocytes and inflammatory cells, focal necrosis and glomeruli enlarged with hyper-cellularity. All tested samples were positive for FAV and sequencing confirmed the FAV-E (serotype 8b) strain, which is 99.53% identical to some isolates from Turkey.