Comparative Assessment of SepsiTest™ Platform to BactScreen™ and " in-house " MGB-based All Bacteria Assay for Detection of Bacteraemia in Whole Blood Samples





Journal title

Libyan Journal of Veterinary and Medical Sciences


Vol. 2 No. 2


Marwan Draid


1 - 8


Several molecular techniques that are commercially described in the literature for early detection of blood stream infection in attempting to overcome the limitations of the gold standard blood culture. SepsiTest™ is aCE marked commercial platform that has been described in the literature. However, there were no studies addressing the accuracy of the test as a diagnostic platform for detection bacteraemia in whole blood samples. The study, conducted to investigate and discuss the strategy of SepsiTest™ in comparisonto our previously validated real-time PCR based assays (BactScreen™) and the previously published “in-house” minor groove binder (MGB)-based all bacteria assay. The three assays showed different sensitivity patterns for detecting bacteria in blood stream pathogens in favouring of using BactScreenTMtest. SepsiTest™ could be valuable but their lowest sensitivity in addition to their use of the unspecific SYBR Green fluoresce dye that question its diagnostic accuracy could be used as a last choice molecular diagnostic technique for detection of bacteraemia in whole blood samples. However, SepsiTest™ strategy rather, may provide useful diagnostic tool for detecting live pathogens in food technology arabic 32 English 130



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