Abstract
Extraction of DNA is one of the most basic and critical steps affecting molecular-based techniques. There are many popular DNA isolation methods from human whole blood for efficient genomic DNA extraction. This study was aimed to compare the genomic DNA quality and quantity between modified chemical Non-Phenolic Non-Enzymatic RapidMethod and commercially available silica column-based blood DNA isolation kit (QIAamp DNA Blood Mini Kit). Genomic DNA was extracted from nineteen female Libyan students using both protocols was evaluated using gel electrophoresis, Nanodrop spectrophotometric analysis, and real time PCR assay. Results revealed significant differences among the two methods (40.55 ± 37.65 ng/μL for modified chemical; 22.84 ± 6.1 ng/μL for QiagenQIAamp Kit; P= 0.04). The DNA purity (OD260/OD280) of DNA obtained from both methods produced the highest purity DNA, with differences being not statistically significant. Our results showed that modified chemical method was the best choice to DNA extraction from whole blood samples in large scale because this method was found cheap enough with good yield over commercial kits especially in the poor laboratories. On the other hand, this method is providing a sufficient quantity and quality of DNA for real- time PCR analysis.