Abstract
Antifungal metabolites, inhibitory to Fusarium culmorum, were isolated from the antagonistic bacteria Pseudomonas fluorescens grown in King’s B liquid medium. The cell-free filtrates obtained, were tested using an agar diffusion assay for their ability to inhibit Fusarium culmorum. The results showed that the cell-free filtrates of P. fluorescens PS02 and PS14 isolates inhibited mycelial growth and conidial germination of Fusarium culmorum. However, only extracts obtained by the solvent diethylether showed clear inhibition zones compared to those extracts obtained by dichloromethane. The Inhibition zones of mycelial growth and conidial germination of Fusarium culmorum were only observed for all the acidic extracts of both bacterial isolates.