CH664 : Cell Biology

Department

Academic Program

Type

General

Credits

Prerequisite

Overview

On completion of this course, students should be able to understand:

· The basic properties of prokaryotic and eukaryotic cells

· The function of the major biological molecules;

· DNA replication, repair, and expression;

· The nature of gene expression and regulation;

· The nature of cell cycle and programmed cell death; and

· The nature of cell signaling;

· The nature of cancer; and the basic concepts of DNA recombinant technology - gene cloning

Intended learning outcomes

A) Knowledge & und understand

أ.1 • Introduce students to the dynamic relationships between cell structure and the biochemical reactions that are necessary for cell growth, differentiation, survival and death with an emphasis on eukaryotic cells.

• Introduce the concepts and underlying principles of prokaryotic and eukaryotic cellular and molecular biology

• Understand the basic properties of prokaryotic and eukaryotic cells; Describe the function of the major biological molecules;

• Account for the structure and function of the eukaryotic cell and its organelles

• account for the genetic information flow in the eukaryotic cell; including nucleic acid structures, the definition of a gene, the organisation of the genome, the replication, the formation of RNA (transcription), the processing of pre mRNA and the protein synthesis (translation).

• Understand DNA replication, repair, and expression;

أ.2 • Account for how genes are regulated

• Account for how cells can communicate and the central intracellular signal transduction pathways.

• Account for intracellular protein transport.

أ.3 • Account for cell motility and regulation of cell form and movement; including cytoskeleton organisation and generation of force and cell motility.

• Describe the cell cycle and the cell division and account for how these are regulated.

• Explain the nature of gene expression and regulation; Understand the nature of cell cycle and programmed cell death;

• Understand the nature of cell signaling

B) Mental skills

· Acquisition of global theoretical and practical concepts around analysis of DNA

· Analyze the nature of cancer

· Discriminate the basic concepts of DNA recombinant technology -gene cloning.

C) Practical & professional skills

· Provide students a background in contemporary biotechnology techniques currently utilized in biological science laboratories

· Describe and carry out basic molecular genetic methods; including work with bacteria, PCR amplification and analysis and electrophoresis of nucleic acid

· Describe and carry out basic cell culture and microscopy.

· A practical hands-on experience in performing cytogenetic and molecular techniques.

· Experimental strategies, pharmacological approach, and epigenetic technologies: immunocytochemistry, chromatin Immunoprecipitation, chromatin reconstitution, RNA ChlP, characterisation of DNA methylation patterns by isoschizomeres and bisulphite sequencing, small RNA cloning, use of epigenetic pathway mutants, detection of genes, transcripts, proteins and protein complexes.

Teaching and learning methods

Lectures
study case
Seminars
Assignments

Methods of assessments

1. جدول التقييم (Assessment table)

رقم التقييم	أساليب التقييم	مدة التقييم	وزن التقييم	النسبة المئوية	تاريخ التقييم (الأسبوع)	ملاحظات
التقييم الأول	Two test exam	6	30	30	4&8
التقييم الثاني	Oral presentation	0	8	8	12
التقييم الثالث	assignments	0	12	12	15
التقييم النهائي	Final Exam	4	50	50	16
التقييم الأول	Two test exam	6	30	30	4&8
المجموع			100 درجة	100%

1. محتوى المقرر (Course contents)

الموضوع العلمي	عدد الساعات	محاضرة	معمل	تمارين	مناقشة	مذاكرة مستقلة
· Introduction to cell & Molecular Biology · Genes, Genomics and Chromosomes.	3	1				5
· Chemical foundations	6	2				5
· Basic Molecular Genetic Mechanisms. · Molecular Genetic Mechanisms · Molecular Genetic Techniques	9	3				5
· Cell Biology Techniques. · Regulating the Eukaryotic Cell Cycle	3	1				5
· Biomembranes. · Transport of ions & small molecules. · Cell energetics	9	3				5
· Integrating Cells into Tissues. · Cell Death –poptosis. · Stem Cell Biology.	6	2				5
· Molecular Biology of Cancer.	3	1				5
· Moving Proteins into Membranes and Organelles. · Vesicular Traffic -The Secretory Pathway & The Endocytosis Pathway.	3	1				5
· Signalling I and II	3	1				5
· Cytoskeleton and locomotion	3	1				5

Heterocyclic chemistry and spectroscop (CH660)
Bio analytical Chemistry (CH661)
Biophysics (CH662)
Cell Biology (CH664)
(CH665)
General Metabolism (CH667)
Experimental Biochemistry (CH670)
Endocrinology & Hormones (CH669)
Conjugated Proteins & Immunochemistry (CH668)
Enzymology and Vitamins (CH666)
Microbial Biochemistry (CH**6)
Biological Fluids (CH675)
Technical Writing (TW600)