Genetic variation of Fusarium solani isolates based on RAPD-PCR analysis.

Date

2013-1

Type

Article

Journal title

Islamic Azad University, Plant Protection Department

Issue

Vol. 1 No. 2

Author(s)

Nuria Ali Elamri
Issa Saleh Farag
Hatem Mostafa Younes

Abstract

Fusarium solani is a fungal pathogen causing citrus dry root rot complex (Bender and Menge, 1984). Genetic variation between isolates of both Fusarium moniliforme and Fusarium solani have been detected using PCR (Huang et al., 1997; Bereneuza et al., 2004). Random amplified polymorphic DNA-Polymerase chain reaction (RAPD-PCR) (Williams et al., 1990; Micheli et al., 1994) was used for the assessment of genetic variability among six isolates of F. solani. Out of ten random primers screened, three gave reproducible polymorphic bands. The amplification products using OPA13, OPD8 and OPD8 were in the range of 396 bp to 4.650 Kb. RAPD fingerprints of F. solani isolates revealed polymorphism in 80% of primer OPE20, followed by 75% OPA13 and 71% of OPD8. Cluster analysis of RAPD-PCR products using these three primers indicated high levels of variations in banding patterns of all isolates tested

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