Abstract
Silver nanoparticles (AgNPs) were synthesized using an environmentally friendly, biosynthetic approach. The aqueous leaf extract of Hyoscyamus muticus, employed for the first time in this capacity, served as a capping ligand for AgNPs formation. To enhance the reducing capability of the extract, sodium borohydride (NaBH 4 ) was added. The synthesized AgNPs exhibited high stability (for 26 weeks) at room temperature and pH 9.1. The AgNPs were characterized using FTIR, UV–VIS, TEM, DLS, and zeta potential analysis. The UV–VIS result shows that the surface plasmon band (SPB) is centered at 389 nm confirming successful preparation of AgNPs. The monodispersed small size (17.3 ± 4.5 nm) occurred based on TEM results. The zeta potential value of AgNPs was ( 64 mV), indicating high stability and excellent dispersion. The stability and size of spherical AgNPs were investigated regarding pH and temperature. The antibacterial activity of produced AgNPs against a Gram- negative bacteria (Acinetobacter baumannii, 17978) and Gram-positive bacteria (Staphylococcus aureus, 6538P) was determined using the disk diffusion method, and Time-kill curves. Green synthesized AgNPs possess good antibacterial activities. These findings will encourage researchers to use these AgNPs as a possible alternative to chemically synthesized, which could be used as antibacterial agents in the future, due to their bio-bacterial properties.