Abstract
ABSTRACT Lepidium sativum Linn. Belonging to the Brassicaceae (Cruciferae) family. It is an annual herb, containing many pharmacologically active phytochemicals which contribute to the treatment of many diseases and symptoms. the main aim of the study is to re-evaluate the phytochemical components of the seed and to study the effect of its constituents on estrogen receptors. Molecular modeling was used to evaluate the pharmacological effects of the seed constituents on the functional ovarian cyst and to confirm the traditional folkloric use of L- sativum seeds as a plant to reduce the size or remove the functional ovarian cyst. Thin-layer chromatography was used to identify and separate the seed constituents using a suitable solvent system. In addition, Spectroscopic methods, UV-visible, fluorescence, and FTIR spectroscopic techniques were used to further evaluate the seed constituents. AutoDock and AutoDock Vina in PyRx virtual screening software were used to study the binding docking energies between many components of Lepidium sativum seeds with α and β estrogen receptors. The results of this study showed that Lepidium sativum seeds contain many phytochemical compounds, such as phytosterols, phenols, flavonoids, and alkaloids, that bind to human estrogen receptors (α1x7E and β1x7J) and reveal a significant pharmacological effect on functional ovarian cysts.