Abstract
The current study was conducted in laboratories of department of Plant Protection, university of Tripoli to evaluate the ability of four Trichoderma isolates namely: T. viride (T1); T. longibrachiatum (T2); T. koningii (T3); T. viride (T4) to antagonize four isolates of the pathogenic fungi Rhizoctonia solani isolated from soil and potato tubers (Solanum tuberosum L.) infected with black scurf disease collected from four areas of Tripoli: Tajourah (R1); Garabouli (R2); Gaser Benghasheer (R3); Janzour (R4). Experiments were conducted as Complete Randomized Design (CRD). Results of mycoparasitism and non-volatile metabolites showed 100% inhibitions of all tested Rhizoctonia isolates treated with (T1). Moreover, T4 isolate caused 80%, 5% inhibition of R1, R2 growth, respectively, in metabolite volatiles experiment. Microscopic studies of anatagonisim showed direct mycoparasitism including: lysis, granulation and coiling of Rhizoctonia by Trichoderma hyphae.