Normal biodistribution of canine leukocytes labeled in whole blood with technetium-99m stannous fluoride colloid and their ability to localize induced inflammatory foci

Date

2010-8

Type

Article

Journal title

The Veterinary Journal

Issue

Vol. 2 No. 185

Author(s)

Mohamed Hamrouni S. Abushhiwa
Nouria S.Salehi
Meir Lichtenstein
Peter M. Lording
Peter J. Finnin
Robert C. Whitton
Jennifer A. Charles
Bruce W. Parry

Pages

157 - 162

Abstract

This study assessed the biodistribution of autologous leucocytes radiolabelled with technetium-99m stannous fluoride colloid (99mTcSnC) for detection of foci of induced inflammation in dogs. Venous blood was collected from seven healthy dogs and incubated with 99mTcSnC for 1 h at room temperature. Radiolabelled samples were injected intravenously (IV) and the dogs were scanned using a gamma camera. Another seven healthy dogs were injected intradermally with tumour necrosis factor α and then IV with 99mTcSnC radiolabelled autologous blood 3 h later before being scanned. The radiolabelled leucocytes localised to sites of inflammation by 30 min post-injection. IV injection of autologous leucocytes radiolabelled with 99mTcSnC appears to be a sensitive method for localisation of induced foci of inflammation in dogs.

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