Genetic and biochemical characterization of a novel metallo-β-lactamase, TMB-1, from an Achromobacter xylosoxidans strain isolated in Tripoli, Libya

Date

2012-1

Type

Article

Journal title

American Society for Microbiology

Issue

Vol. 5 No. 56

Author(s)

Allaaeddin El Salabi

Pages

2241 - 2245

Abstract

An Achromobacter xylosoxidans strain from the Tripoli central hospital produced a unique metallo-beta-lactamase, designated TMB-1, which is related to DIM-1 (62%) and GIM-1 (51%). blaTMB-1 was embedded in a class 1 integron and located on the chromosome. The TMB-1 -lactamase has lower kcat values than both DIM-1 and GIM-1 with cephalosporins and carbapenems. The Km values were more similar to those of GIM-1 than those of DIM-1, with the overall kcat/Km values being lower than those for GIM-1 and DIM-1

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