• Using analytical chemistry methods in the classroom and identifying biological molecules.• The use of chromatographic and spectral analysis methods in the analysis of biomolecules.• How to prepare and process samples before bioanalysis.• The student gains experience in different analysis methods, DNA and RNA, and learns about the principle of quality in the methods and results, and using Microsoft Excel in processing the results.• How to apply the electrolysis methods used in bioanalysis, including the various biosensors.
Intended learning outcomes
a. Knowledge & understandingA.1 The student will be familiar with advanced analytical methods and their application in analyzing different biological molecules.A.2 Giving the student a strong experience in installing and programming devices.A.3 The student learns the skills of dealing with biological samples and preparing them for analysis. A.4 Learn methods of analyzing and processing analysis results using Microsoft Excel. A.5 The student will be familiar with the use of the principle of quality in bioanalytical chemistry and standard criteria for the results of different biological samples.B. Mental skillsB.1 Analyze problems and apply technical solutions for analyzing different biomolecules. B.2 The ability to compare different devices and their efficiency used in biological analysis.B.3 The ability to identify the advantages and disadvantages of biomolecular analysis.B.4 The ability to express and process the quality of the results and the ability to participate in research activities related to the analysis of various biological molecules.B.5 The ability to use Microsoft Excel to analyze results. T. Practical & professional skillsC.1 Using modern chemical analysis methods in preparing and separating biological samples before analysis.C.2 The use of automatic analysis devices such as spectroscopy, chromatography, and electrophoresis in the analysis of biological molecules.C.3 The ability to handle practical errors during measurement with these devices.C.4 The ability to distinguish between qualitative, quantitative and instrumental bioanalytical methods and use each of them and the skill of using ISO and quality in selecting bioanalytical methodsC.5 The ability to use mathematical and statistical methods to analyze the results obtained using Microsoft Excelw. Generic and transferable skillsD.1 presents information and explains the mechanism of action of all biological analysis methods, orally and in writing D.2 Using appropriate techniques for automatic analysis of biomolecules (real samples)D.3 Work in an individual and independent manner, relying on personal skill to understand and analyze all the methods usedD.4 acquires skills in analyzing different biological molecules and their quality and use in bioanalytical chemistryD.5 Discussion in a scientific manner and can write reports in sound scientific language and be able to present data and use Microsoft Excel to process results
Teaching and learning methods
Lectures
Assignments
Queses
Methods of assessments
Written test (choice style essay {closed book}) 60%
Panel discussions and presentation of topics 40%
1. محتوى المقرر (Course contents)
الموضوع
العلمي
عدد الساعات
محاضرة
تمارين
مناقشة
مذاكرة
مستقلة
الاسبوع
Biological
Buffers
Quantitative
and qualitative Instrumental
Measurements
Solubility ,Permeability Ionic strength
Dependence of pKa value , Complex formation ,
Inert substances , UV absorption
Purity – simple method of manufacture , Costs
Overview of most important properties of bio buffers,
product list of biological buffers
Charge Balance in Buffers
How Bio buffers works
Physiology
Pathology
Problems
2
2
-
-
-
1
Spectroscopic Methods
Total
Protein
Lowry
Method
Smith(BCA)Method
Bradford
Method
.Ninhydrin-Based Assay
Other
Protein Quantitation Methods
Total
DNA
Diamino
benzoic Acid(DABA)Method
Diphenylamine(DPA)Method
Other
Fluorometric Methods
Total
RNA
Total Carbohydrate
Ferricyanide Method
Phenol-Sulfuric
Acid Method
Purpled
Assay for Bacteria Polysaccharidesction.
4
4
-
-
-
2
Electrophoresis of Biomolecules
Electrophoretic
Support Media
Paper
.Starch Gels
Polyacrylamide
Gels
.Agarose Gels
.Polyacrylamide-Agarose Gels
Effect
of Experimental Condition On electrophoretic Separations
Electric
Field Strength Gradients
Pulsed
Field Gel Electrophoresis(PFGE)
Detection
of Proteins and Nucleic Acids After Electrophoretic
Separation
Detection
of Enzymes by Substrate Staining
The
Southern Blot
The
Northern Blot
The
Western Blot
Detection of DNA Fragments on Membranes with
DNA
RNA Electrophoresis
Applications
of Zone Electrophoresis
Isoelectric
Focusing and 2D Electrophoresis
Capillary
Electrophoresis
The
capillary electrophoresis separation techniques
Capillary
electrophoresis among other liquid phase separation methods
Basic
instrumentation for liquid phase separations
CE
versus HPLC from the point of view of Green Analytical Chemistry
CE
as a method of choice for portable instruments
World-to-chip
interfacing and the quest for a ‘killer’ application for LOC devices .
Gradient
elution moving boundary electrophoresis and
electrophoretic
exclusion
Possible
ways of surmounting the disadvantages of CE
Sample
preparation in CE
Is
capillary electrophoresis a green alternative
Capillary
Electrophoresis
Capillary
electrophoresis -HPLC-MS
Applications
in Biochemistry
Mass
Spectrometry of Biomolecules
Problems
Experiments
6
6
-
-
-
3
Nucleic
Acids Amplification and Sequencing
CsCl
Density Gradient Centrifugation
Total
Cellular DNA Isolation
RNA
Isolation – The Proteinase K method
Nucleic
Acid Amplification – The Polymerase Chain
Reaction
(PCR)
The
Principle of PCR
The
Rate of Amplification During a PCR
Reagents
for PCR
Real-Time
PCR
Reverse
Transcription – PCR (RT-PCR)
Nucleic Acid Sequencing 156
The Use of Restriction Enzymes in Sequencing
The Chemical Cleavage method
(The
Maxam-Gilbert method)
The
Chain Terminator method (The Sanger or
Dideoxy
method)
RNA
Sequencing
Protein
Sequencing
End-group
Analysis 1
N-terminal Analysis (Edman
Degradation)
C-terminal Analysis
Disulfide Bond Cleavage
Separation and Molecular Weight
Determination of the Protein
Subunits
Amino Acid Composition
Cleavage of Specific Peptide Bonds
Enzymatic Fragmentation
Chemical Fragmentation Methods
Sequence Determination
Ordering of Peptide Fragments
Determination of Disulfide Bond Positions 1
Protein Sequencing by Mass Spectrometry
4
4
-
-
-
2
Bio Molecular Recognition
Antibodies
Bioassays
Biosensors
DNA Arrays
Pyrosequencing
Quantitative Immunoassays with Labels
Micro-TAS, Lab-on-a-Chip, and Microarray
Device
2
2
-
-
-
1
Chromatography
of biomolecules
Application
of HPLC-MS and HPLC-MS-MS for
bioanalysis
Reversed
Phase Liquid Chromatography
Ion
Exchange Chromatography
Affinity
Chromatograph
Size
Exclusion Chromatography
Ion
chromatography in bioanalysis
4
4
-
-
-
2
1.
Validation of New Bioanalytical Methods
2.
Opener:
The Need for Quality Assurance
Basics of Quality Assurance
Control Charts
Method Validation
The Horwitz Trumpet: Variation in Inter laboratory Precision
Standard Addition
Internal Standards
Efficiency in Experimental Design
3.
Using
Microsoft Excel in Advanced
bioanalytical Chemistry Problems
