CH667 : General Metabolism

أ.1

Describe basic metabolic pathways, anabolic and catabolic.

أ.2

Use enzyme kinetics to evaluate enzyme activities and their regulation.

أ.3

Describe the principles of metabolic regulation.

أ.4

Assess the crucial role of some hormones with regard to the integration of metabolic pathways.

أ.5

Understand the molecular basis of metabolic diseases.

ب.1

Recognize how the catabolic breakdown of the substances is associated with release of free energy; whereas, free energy is utilized during synthesis of biomolecules i.e., anabolic pathways

ب.2

Apply concepts of enzymology to evaluate how the metabolism is coordinated

ب.3

Evaluate the importance of allosteric regulation for controlling metabolic flux

ب.4

Acquire knowledge related to the role of TCA cycle in central carbon metabolism importance of anaplerotic reactions and redox balance.

ب.5

Describe the basic energy metabolism of cells

ج.1

Distinguish between exergonic and endergonic reactions in terms of available energy change.

ج.2

Apply the knowledge of metabolic pathways to biochemical research.

ج.3

Ability to critically evaluate a problem and resolve to challenge blindly accepted concept.

ج.4

Ability to analyze texts, evaluating ideas and scientific strategies.

ج.5

Provide an illustration of the link between experimentation and scientific understanding.

د.1

Writing a research report: This will be important for any career in research.

د.2

Analyze the data and submit.

د.3

Communicate ideas and arguments effectively.

د.4

Manage time and resources effectively and set priorities.

د.5

Perform self-directed learning.

رقم التقييم

أساليب التقييم

مدة التقييم

وزن التقييم

النسبة المئوية

تاريخ التقييم (الأسبوع)

ملاحظات

التقييم الأول

Two test exam

6

30

30

4&8

عددها 2

التقييم الثاني

Oral presentation

0

8

8

12

عددها 2

التقييم الثالث

Assignments

0

12

12

15

عددها 3

التقييم النهائي

Final Exam

4

50

50

16

المجموع

100 درجة

100%

الموضوع العلمي

عدد الساعات

محاضرة

معمل

تمارين

مناقشة

مذاكرة مستقلة

عدد الاسابيع

Overview of metabolic pathways

4

2

1

3

1

Bioenergetics:Laws of Thermodynamics - first and second law. Concept of enthalphy, entropy and free energy. Standard free energy. Endergonic and exergonic reactions. Coupled reactions. High-energy compounds – structural features of ATP and its free energy change during hydrolysis, other high energy compounds.

8

4

1

3

2

Biological oxidation: Ultra structure of mitochondrion, electron transport chain. Electron transport complexes Complex I, II, III and IV. Uncouplers and inhibitors of respiration (Rotenone, antimycin. cyanide and 2,4 DNP). Oxidative phosphorylation, P/O ratio. Formation of ATP-Outline of Mitchell’s hypothesis. Substrate level phosphorylation with examples.

8

4

1

3

2

Metabolism of carbohydrates: Glycogen metabolism – glycogenolysis, glycogen synthesis. Glycolysis, energetic of glycolysis. Entry of other carbohydrates into glycolytic pathway. Fates of pyruvate – conversion of pyruvate to lactate, alcohol and acetyl Co-A. Citric acid cycle and it’s energetic. Amphibolic integrating roles of TCA cycle. Anaplerotic reactions. Pentose phosphate pathway and its significance. Cori cycle. Gluconeogenesis.

8

4

1

3

2

Metabolism of lipids: Oxidation of fatty acids – α, β and ω types, β-oxidation of even number saturated fatty acids. Energetics of β-oxidation. Biosynthesis of even number saturated fatty acids. Ketone bodies formation. Outline of cholesterol biosynthesis

8

4

1

3

2

Metabolism of amino acids:General reaction of amino acid degradation – Transamination, deamination and decarboxylation. Ketogenic and glucogenic amino acids. Urea cycle and its significance.

8

4

1

3

2

Interrelationships of carbohydrate, protein and lipid metabolism

4

2

2

1

Xenobiotics and their toxicity- Antagonism and synergism-Xenometabolism (phase I and phase II reaction)

4

2

2

1

Glucuronidation, conjugation, glycosylation, methylation and acylation reactions for Xenobiotics including drugs

4

2

2

1