MB334 : General Microbiology

A.1

Describe the morphology and structure of microorganisms.

A.2

Summarize basic microbial genetic principle, and analyze consequences of mutation, and genetic recombination.

A.3

Identify microbial physiology and metabolism.

Understand the microbial metabolic pathways in general terms.

A.4

Knowledge on the microbial infectious diseases, and List different virulence factors of infectious microbial.

B.1

Asses appropriate methods for isolation, and identification of infectious agents.

B.2

Describe the most important methods of decontamination, and principles of infection control.

B.3

Read sensitivity test on the culture plates.

B.4

Describe the basics of antimicrobial uses, and resistance.

B.5

Operate different laboratory procedures in analysis of biologicalsamples.

C.1

Observe the principles of isolation and identification of bacteria (with special reference to principles of staining methods)

C.2

Identify medical important bacteria based on microscopic examination.

C.3

Perform a Gram stain, Ziehl- Neelsen stain, and identify according to morphology.

C.4

Identify culture media, and biochemical tests commonly used forbacterial.

D.1

Participate actively in teamwork.

D.2

Appreciate team work in seminar.

D.3

Interpret microbiological reports.

D.4

Formulate a systematic approach for laboratory diagnosis of common infection.

D.5

Follow measure for infections control.

s.n.

Assessment Method

Due (week)

Weight (%)

Notes

1

Midterm exam I

The eighth

15

At the end of first term

2

Midterm exam II

Weeksixteen

15

At the end of second term

3

Practical exam

Week seventeen

30

At the end of semester

4

Final exam

Week eighth teen

40

At the end of semester

5

Oral exam

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/

-

6

Assignments/HWs

-

-

During the semester

Total (%)

100 %

Topics&Practical

Total hrs.

Lectures

Lab

Ex/Hw

1.

The diversity of microbiology- General differences among bacteria, fungi, algae, protozoa, and viruses, Branches of Microbiology Based on the Type of Microorganism, History of Microbiology.Lab: Introduction to the microbiology laboratory-Safety rules and aseptic techniques.

4

2

2

-

2.

Prokaryotic Anatomy& Differences between Prokaryotic ,and EukaryoticLab: Microscope and laboratory instruments: -Use and care of light microscope and preparation of specimen for microscopic observation. Autoclave, Hot Air oven, Incubator, and Anaerobic jar eta...

4

2

2

-

3.

Bacterial anatomy & morphology: Structure of bacteria cell, Bacterial Shapes, Arrangements, and Sizes. Lab: Preparation and staining of specimens for light microscopy: Smear preparation, dyes and simple staining.

4

2

2

-

4.

Bacterial classification: Taxonomy of bacteria, Nomenclature, Identification. Microorganisms. Lab: Culture Media - defined media, commonly used media, and types of media- liquid semi-solid, solid, selective, differential and enrichment.

4

2

2

-

5.

Bacterial metabolism & growth:Requirements for Growth, Bacterial Division Replication, and Reproduction, Bacterial Growth Curve cont. Lab:Culture Media- Preparation of culture media.

4

2

2

-

6.

Microbial metabolism:Structure of enzyme, classification of enzyme, mechanism of enzyme action. Oxidation, reduction. & fermentation.Lab: Microbial cultivation and isolation of pure culture- methods

of pure culture of microorganisms – spread plate method.

4

2

2

-

7.

Bacterial Genetics : The bacterial genome (chromosome, extrachromosomal elements, bacteriophages), gene organization (operons, citron's), DNA replication (binary fission, replication rules & fidelity), polymerase chain reaction.

Lab:Simple staining.

4

2

2

-

8.

Midterm Exam I

9.

Bacterial Genetics – Genes, Maintenance & Exchange: Mutation & repair (types of mutations, mutagens, DNA repair processes), gene transfer (transposons, extrachromosomal elements, mechanisms of DNA exchange, recombination, genetic engineering, etc).

Lab: Differential staining- Gram staining.

4

2

2

-

10.

Microbial control : Terminology of microbial control - Sterilization, disinfection, antisepsis & sanitation .Antibiotics and chemotherapeutics. Mechanism of action. Antibiotic resistance. Lab: Methods disinfection & methods sterilization.

4

2

2

-

11.

Nosocomial infection: Definition, Microorganisms in Hospital, Compromised Host, Chains of Transmission, and Control of Nosocomial infection.Lab: Hand washing, aseptic techniques.

c-

4

2

2

-

12.

Microbial mechanism of pathogenicity: Infection and disease, Normal flora, and Opportunistic. Virulence factors.

Lab: Antibiotic susceptibility tests- Disk diffusion tests, and broth dilution tests (MIC and MBC).

4

2

2

-

13.

Introduction to the Virology.

4

4

2

2

2

2

-

-

14.

Introduction to the Mycology.

4

2

2

-

15.

Introduction to the Parasitology.

4

2

2

-

16.

Midterm Exam II

17.

Practical Exam

18.

Final Exam