HO4834 : Advanced plant tissue culture

Department

Department of Gardening

Academic Program

Master in Horticulture

Type

University requirement

Credits

04

Prerequisite

Overview

A general advanced course for all master's students in the department, starting with a general introduction to tissue culture techniques and their historical development and related terminology with a review of problems, advantages and applications, establishing and planning laboratories, nutrient media, their components, types, preparation and sterilization - the effect of nutrient medium components on reproduction and growth of explants, reproductive systems in farms Histology - organ formation, somatic embryo formation and its pathways, physiological and biological changes associated with the formation of somatic embryos, culture of zygotic embryos - protoplast culture and cellular hybridization, callus cultivation and production of secondary compounds, Bioreactors and their use in micropropagation, preservation of genetic assets - phylogenetic variation - reproductive systems used in genetic engineering - a study of scientific papers covering these topics. It concludes with various topics: flowering in tissue cultures - the quality of tissue plants - integration

Intended learning outcomes

Successfully completing this course, the student will be able to:

Demonstrates accurate information about different tissue culture techniques.

2. Can supervise and manage research and commercial tissue culture laboratories.

3. Distinguish between the types of problems facing the exact propagation of any type of horticultural plants.

4. Differentiate between accurate calculations of materials used in tissue culture

5. Determine the different plant parts that are used in tissue culture and the most successful ones

Teaching and learning methods

1- Lectures

2- Reviewing scientific papers on various topics

3- Collecting information by the student

4- Preparing a term paper

Do a mini research and make an offer

Methods of assessments

Evaluation methods

evaluation date

First midterm exam

after the fifth week

Second midterm exam

after the tenth week

Scientific activities (e.g. writing a report or giving a presentation)

during the semester

Theoretical final exam

after the fifteenth week