CH451P : Biochemistry Practical

Department

Department of Chemistry

Academic Program

Bachelor in Chemistry

Type

Compulsory

Credits

01

Prerequisite

CH451

Overview

The practical side involves getting to know the following:

First, qualitative (descriptive) ways to identify amino acids.

- An overview of the nature of amino acids, their chemical qualities.

Second, the practical aspect:

1- General detection of amino acids, nindehydrin reagent.

2- Detection of aromatic amino acids, including: · Zantoprotic test. · Mellon test. · Hopkins-Cole test.

3- General detection of basic amino acids such as arginine, Sakaguchi detection test.

4- General detection of sulfur amino acids.

-Testing for sulfur amino acids such as: Cysteine, Nitroprusid reagent,

- Third, qualitative (descriptive) ways to distinguish between different sugars.

1- Molish test to distinguish between carbohydrates and others.

2- Iodine test to detect polysaccharides.

3- Benedict test for reducing sugars.

4- Barfoid test to distinguish between mono&di reducing sugars.

5- Bials test to distinguish between hexoses and pentoses.

6- Selvanov's test to distinguish between aldosis and ketosis.

7- Ozazone test to distinguish between different pellets.

- Estimation of some important components in the blood.

1- Estimation of the amount of hemoglobin.

2- Estimation of the amount of plasma proteins (albumin / globulin).

3- Estimation of the amount of glucose in the blood.

4- Estimation of the amount of cholesterol in the blood (serum).

5- Estimating the amount of urea in the blood.

Intended learning outcomes

1. Introducing the student to occupational safety procedures in chemical laboratories.

2. Enable the student to prepare solutions and chemicals that he will work with.

3. Enable the student to use some simple equipment and devices.

4. Teaching the request methods of detection of some compounds such as various amino acids.

5. Enable the student to know how to distinguish between different sugars.

6. The student learns how to deal with materials and equipment inside the laboratory.

7. The student demonstrates how to use and calibrate the devices.

8. The student has multiple methods of preparing different solutions.

9. The student determines how to handle the samples during the analysis.

10. Compare different methods of performing a particular analysis and calculating the results of the analysis.

11. Discusses the nature and danger of chemicals.

12. Coercion and analyze the reasons for choosing the appropriate method for the preparation of chemical solutions.

13. Analyzes the results of experiments.

14. Discovers how important it is for the results of the analyzes to be compatible with realistic values.

15. Compares analysis methods with standard specifications.

16. Writes reports on the results of chemical analyzes accurately and skillfully.

17. Uses appropriate methods to perform analysis of a particular substance.

18. Uses international specifications and compare analysis methods with them.

19. Employs his knowledge of methods of determining the quality of industrial products.

20. Uses devices and equipment with high efficiency in the laboratory.

21. Ability to self-learning.

22. Ability to learn continuously.

23. Ability to use information technology in research and professional development. 24. Ability to work in a team. Respect for professional ethics.

Teaching and learning methods

1- Granting the student, a commitment to all the required experiments and methods of preparing solutions.

2- Laboratory activities and information collection.

Real-time follow-up during the experiments to ensure that each student is able to use the tools correctly

Methods of assessments

Notes	Percentage	Evaluation weight	Evaluation duration	Evaluation methods	No.
	15%	15	Fifth week	First written test (multiple choice style and essay questions)	1
	15%	15	Tenth week	First written test (multiple choice style and essay questions)	2
	10%	10		Reports on experiments	3
	10%	10		Attendance and experiments conductance	4
	50%	50	Last week	Final Exam	5

Course Content

Week	Scientific topic	Teaching hours	Lectures	Exercises	Discussion
1	First, qualitative (descriptive) ways to identify amino acids - An overview of the nature of amino acids, their chemical properties	3
2	Second, the practical aspect: 1- General detection of amino acids, nindehydrin reagent.	3
3	2- Detection of aromatic amino acids · Xantobrotec Test · Mellon test · Hopkins Test – Cole	3
4	3- General detection of basic amino acids such as arginine, Sakaguchi detection test	3
5	First med term exam
6-7	4- General detection of sulfur amino acids · Testing for sulfur amino acids such as cetien · Nitroprusside reagent	6
8-9	Third: qualitative (descriptive) ways to distinguish between different sugars 1- Molish test to distinguish between carbohydrates and others 2- Iodine test to detect ammonia and dextrins 3- Benedict test for reducing sugars 4- Barnwid test to distinguish between hexagons and ketoses 5- Bials test to distinguish between hexagons and ketosates 6- Selvanov's test to distinguish between aldozate and ketosis	6
10	Second med term exam
11,12,13	Estimation of some important components in the blood 1- Estimation of hemoglobin quantity 2- Determination of the amount of plasma proteins (albumin / globulin) 3- Estimating the amount of glucose in the blood 4- Estimation of blood cholesterol (serum) 5- Estimating the amount of urea (urea) in the blood	6
14	Final exam
	Total	30

References

Reference Title	Publisher	Version	The author	Reference location
Bioanalytical Chemistry	A JOHN WILEY & SONS, INC., PUBLICATION		Susan R. Mikkelsen & Eduardo Corto´n

Quranic Studies 1 (AR101)
Principles of statistics (ST100)
Fundamentals of Education (EPSY101)
General Psychology (EPSY 100)
General Chemistry I (.CH101)
Arabic language 1 (AR103)
Arabic language 2 (AR104)
computer 1 (CS100)
General English1 (EN100)
General Teaching Methods (EPSY 201)
Developmental Psychology (EPSY 203)
Quranic studies2 (AR102)
General Mathematics 1 (MM111)
General chemistry II practical (CH102P.)
General chemistry II (CH102.)
Basics Of Curriculums (EPSY 202)
Educational Psychology (EPSY 200)
Computer 2 (CS101)
Arabic language 3 (AR105)
Physical Chemistry I (CH241.)
Analytical Chemistry I (CH211.)
Organic chemistry 1 (CH231.)
Inorganic Chemistry I (CH221.)
Organic Chemistry 2 (CH232.)
Physical Chemistry 2 (CH242.)
Methods of teaching chemistry (CH201)
General Mathematics 2 (MM112)
Research Methods (EPSY301)
Measurements and Evaluation (EPSY 302)
Analytical Chemistry 2 (CH212.)
Inorganic Chemistry II (CH222.)
Organic chemistry 1 Practical (CH231P.)
Physical chemistry 1 Practical (CH241P.)
Inorganic chemistry I Practical (CH222P.)
Analytical chemistry 1 Practical (CH211P.)
Teaching learning Aids (EPSY 303)
Psychological Health (EPSY 401)
Analytical chemistry 3 (CH311.)
Inorganic chemistry 3 (CH321.)
Organic chemistry 3 (CH331.)
Physical chemistry 3 (CH341.)
(CH212P.)
Analytical chemistry 4 (CH312.)
Inorganic chemistry 2 practical (CH321P.)
Inorganic chemistry 4 (CH322.)
Organic chemistry 2 practical (CH331P)
Organic chemistry 4 (CH332.)
Organic chemistry 3 practical (CH332P)
Physical chemistry 2 practical (CH341P.)
Physical chemistry 4 (CH342.)
Physical Chemistry 3 practical (CH342P)
Physical chemistry 5 (CH343.)
Inorganic chemistry 3 practical (CH322P.)
Biochemistry (CH451)
Industrial chemistry (CH441E)
Teaching Application (CH202)
Nuclear chemistry (CH436.)
Analytical chemistry 3 practical (CH311P)
(CH403.)
Biochemistry Practical (CH451P)
Teaching Practice (EPSY 402)
Environmental Chemistry (CH411E)